Fig. 2

Molecular characteristics of identified subpopulations. a Flow cytometric analysis of K562 cells for CD24, GATA1, and GATA2. Right panels: CD24 correlates with GATA1 (R² = 0.68) and GATA2 (R² = 0.44). b Representative histogram FACS plots of the re-analysis of K562 cells for GATA1 (left) and GATA2 (right) after sorting for CD24. CD24^hi sorted population is labeled red, CD24^lo sorted population is labeled blue, isotype control gray. Mean fluorescent intensity (MFI) 2565 for GATA1 high, 2098 for GATA1 low, 2930 for GATA2 high, and 2457 for GATA2 low. c ATAC-seq of CD24^hi and CD24^lo sorted K562 cells (replicates); 2757 peaks are differentially regulated with a fold change of 1.5 and p value <0.001. Blue represents genomic locations less accessible, red locations with higher accessibility compared to the mean of all samples. d Representative UCSC genome browser tracks of open chromatin regions in K562 CD24^hi sorted cells (upper track, red) and K562 CD24^lo sorted cells (lower track, blue). Example regions shown are the GATA2 and CD24 locus. e Gene Ontology term analysis of chromosomal regions, which are more accessible in the CD24^hi population. f Enrichment of ATAC-seq peaks more open in CD24^hi (top) or CD24^lo (bottom) in K562 and hematopoietic stem cell ChIP-seq datasets. Shown are odds ratios calculated using Fisher’s exact test. Values below zero demonstrate de-enrichment (blue) and above zero enrichment (orange). g Overlap of ATAC-seq peaks more accessible in CD24^hi (red) or CD24^lo (blue) with DNAse peaks across 72 different cell types. Left: Number of cell types with overlap is quantified. Right: The different cell types are shown; K562 and CMK leukemia cell lines are highlighted in green