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Fig. 5 | Genome Biology

Fig. 5

From: Patterns of ribosomal protein expression specify normal and malignant human cells

Fig. 5

Differentially expressed RPs display distinct regulatory fingerprints. a–c Expression level of STAT4 (a), GATA1 (b), and CREB1 (c) across the different hematopoietic cell types. Lymphoid and myeloid cell types are colored in black and gray, respectively. STAT4 (P < 0.001) and GATA1 (P < 0.01) show an expression bias toward lymphoid and erythroid cells, respectively. Conversely, the expression of CREB1 is not significantly different between lymphoid and myeloid cell types (P = 0.55). d–f Differences in the activities of STAT4 (d), GATA1 (e), and CREB1 (f) across hematopoietic cell types, as inferred from the regulation of their respective targets. STAT4 and CREB1 show a higher activity in lymphoid compared to other cells (P = 0.09 and P < 0.001, respectively), whereas GATA1 shows a greater activity in erythrocytes (P < 0.01). The distributions of expression levels and activities of transcription factors between cell types of different lineages were compared using the non-parametric Mann-Whitney U test (one-tailed). g The heatmap of the predicted transcription factor binding site (TFBS) motif scores (rows) in the promoters of the three RP genes (columns) that had the highest variance across the different primary hematopoietic cells indicates largely non-overlapping transcription regulatory interactions. h Heatmap of the average activity scores across cell types belonging to the three different lineages (lymphoid, myeloid, and erythroid) for the TFs listed. i TF-ChIP-inferred binding scores of three different lineage-specific TFs (ATF1, TFAP2C, and YY1 for lymphoid, myeloid, and erythroid lineages, respectively) in the promoters of three different RPs displaying lineage-specific expression (RPS29, RPS27L, and RPS3A for lymphoid, myeloid, and erythroid lineages, respectively)

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