Fig. 1

Data for a 2D read of a full-length λ phage dsDNA from the MinION nanopore sequencer. a Steps in DNA translocation through the nanopore: (i) open channel; (ii) dsDNA with lead adaptor (blue), bound molecular motor (orange) and hairpin adaptor (red) is captured by the nanopore; capture is followed by translocation of the (iii) lead adaptor, (iv) template strand (gold), (v) hairpin adaptor, (vi) complement strand (dark blue) and (vii) trailing adaptor (brown); and (viii) status returns to open channel. b Raw current trace for the passage of the single 48-kb λ dsDNA construct through the nanopore. Regions of the trace corresponding to steps i–viii are labeled. (c) Expanded time and current scale for raw current traces corresponding to steps i–viii. Each adaptor generates a unique current signal used to aid base calling