Fig. 7

Characterisation of retrograde response in fission yeast. a Blocking ETC activity results in higher fermentation with similar growth rate. Growth rate curves (solid lines) and glucose concentration in the medium (dotted lines) for wild-type (WT) and antimycin-treated cells as indicated over time. b Venn diagrams of genes induced or repressed >1.5-fold in rpm1 mutants, reb1 mutants, and antimycin-treated cells (ANT). c OXPHOS genes are repressed in response to mitochondrial dysfunction. Heat maps showing 40 repressed (orange, left) and nine induced (green, top right) genes present in overlap from b. Colour intensities represent fold changes as in indicated in key. Additional panels show the 14 repressed (middle) and 19 induced (bottom right) genes differentially expressed in only two of three experiments. OXPHOS genes are marked by blue dots. d Genes of complex 2 are not repressed in response to mitochondrial dysfunction. Box plot showing fold changes of transcript levels encoding OXPHOS complexes and Nde1, in rpm1 and reb1 deletions and antimycin-treated cells as indicated