Fig. 5

Acetyl-CoA synthesis pathways. a Two possible pathways that link glucose metabolism with acetyl-CoA synthesis. b Total proteins from wild-type (WT) cells or the different deletion mutants as indicated were tested for levels of histone H3 lysine 9 acetylation (H3K9), with actin (Act1) levels used as loading control. c Growth of indicated deletion mutants was compared to wild-type cells (WT) in YES medium. d Deletion of the mce1 gene results in partial lysine auxotrophy. Growth of indicated deletion mutants compared to wild-type cells (WT) in EMM medium without (left) and with (right) lysine. e The acs1 gene was replaced by the hygromycin marker in the diploid strain, followed by tetrad dissection on YES medium (four examples shown); all growing spore colonies were sensitive to hygromycin (not shown), revealing that acs1 is essential. f Cells growing on YES media (T0) and then transferred to water completely lost histone acetylation after about 45 minutes (T1). This effect was suppressed if water was supplemented with glucose (glu) or acetate (ac). Total proteins were extracted and levels of H3K9 were tested, with actin levels (Act1) used as loading control