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Fig. 2 | Genome Biology

Fig. 2

From: Functional and regulatory profiling of energy metabolism in fission yeast

Fig. 2

Identification of respiratory deficient mutants. a Fission yeast libraries of non-essential deletion mutants in auxotroph and prototroph genetic backgrounds were arrayed on solid media containing glycerol (GLY) or galactose (GAL) (respiratory conditions) or glucose (fermentative reference condition). Prototroph strains were also tested on respiratory media in the presence of inhibitors of oxidative phosphorylation: antimycin A (ANT) or 2,4-dinitrophenol (DNP). Colony size ratios between respiratory and fermentative media were calculated for each mutant. Colony size ratio distributions for clusters P and A (see main text) are presented separately as box plots. b Overlap between results of all genetic screens. Only mutants with colony size ratios (respiratory/fermentative media) of <0.85 were considered. c Venn diagram of genes from clusters A and P, genes interrogated in our genetic screens whose orthologs affect respiration in budding yeast (S. c.) and genes annotated to affect growth on glycerol in fission yeast (S. p.) (FYPO:0001934), only considering the 47 of 68 genes for which data were obtained in our screens. d Distribution of colony size ratios for genes that affect respiratory growth only in S. pombe (green) or in S. pombe and S. cerevisiae (orange); medians of the two datasets are indicated on top in green and red, respectively. The proportion of genes encoding mitochondrial proteins for the two lists is indicated at the bottom

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