Table 1 Complexity reduction approaches to cloning-by-sequencing in barley and wheat
From: Rapid gene isolation in barley and wheat by mutant chromosome sequencing
Complexity reduction | Technology | Sequence costs | Sequence bias | Data handling |
|---|---|---|---|---|
Chromosome flow sorting | Complex but optimised for >25 plant species, including barley and wheat | Medium | None | Large data set |
Exome capture | Available in several labs | Low | Dependent on reference gene annotation | Small data set |
Transcriptome | Simple (depending on tissue sample) | Medium (expression level dependent) | Target gene has to be expressed in sample; mutations must be in transcript | Large data set; de novo assembly problematic in polyploid wheat |
None | Simple | Very high | None | Very large data set |