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Table 1 Complexity reduction approaches to cloning-by-sequencing in barley and wheat

From: Rapid gene isolation in barley and wheat by mutant chromosome sequencing

Complexity reduction Technology Sequence costs Sequence bias Data handling
Chromosome flow sorting Complex but optimised for >25 plant species, including barley and wheat Medium None Large data set
Exome capture Available in several labs Low Dependent on reference gene annotation Small data set
Transcriptome Simple (depending on tissue sample) Medium (expression level dependent) Target gene has to be expressed in sample; mutations must be in transcript Large data set; de novo assembly problematic in polyploid wheat
None Simple Very high None Very large data set