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Fig. 1 | Genome Biology

Fig. 1

From: Rapid gene isolation in barley and wheat by mutant chromosome sequencing

Fig. 1

Germplasm structuring and gene isolation by MutChromSeq. a Mutagenesis of wheat seed with ethyl methanesulfonate (EMS), screening for mutant plants in the M2 generation and progeny testing in the M3 generation. Additional genetics may be required for some traits to determine complementation groups. b Preparation of liquid suspensions of mitotic chromosomes from M3 roots and labelling of chromosomes with fluorescently labelled DNA probes followed by flow sorting based on DNA content and fluorochrome signal. Pure chromosome preparations are amplified by phi DNA polymerase. c Sequencing of wild type and mutant chromosomes, sequence comparison, and candidate gene identification

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