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Fig. 3 | Genome Biology

Fig. 3

From: Estimating the population abundance of tissue-infiltrating immune and stromal cell populations using gene expression

Fig. 3

Quantitative validation of MCP-counter estimates. a Design of the in vitro RNA mixture validation experiment. b Correlation of MCP-counter scores to samples’ known RNA proportions in RNA mixture samples. The dashed line represents the least-square regression line. The red dots correspond to limits of detection (average score of non-hematopoietic discovery MCP samples on the x-axis and corresponding mRNA fraction predicted by this linear regression on the y-axis). c Endothelial cells and fibroblasts were tested in the same fashion as in b but using qPCR data. HUVEC human umbilical vein endothelial cell. d Three-dimensional scatterplot showing the relationship between the cytotoxic lymphocyte MCP-counter score and T and NK cell proportions in the mixtures. e Correlation of MCP-counter scores with corresponding cell densities measured by immunohistochemistry

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Genome Biology

ISSN: 1474-760X

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