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Fig. 6 | Genome Biology

Fig. 6

From: Time-resolved dual RNA-seq reveals extensive rewiring of lung epithelial and pneumococcal transcriptomes during early infection

Fig. 6

Adherent S. pneumoniae repress epithelial innate immune responses. a At 60 mpi, 124 epithelial genes were significantly repressed upon exposure to ∆cps2E bacteria compared with wild-type pneumococci. b GO term enrichment analysis of 60-mpi repressed genes showed enrichment of “oxidation reduction” (11 genes, p = 4.2 × 10–2), “humoral immune response” (six genes, p = 2.0 × 10–2) and “quinone metabolic process” (six genes, p = 6.0 × 10–7) among others. c ∆cps2E-exposed epithelial cells expressed 2.8 ± 1.3-fold less CXCL8 and 3.0 ± 1.2-fold less DEFB1 than epithelial cells exposed to wild type pneumococci. d We validated CXCL8 and DEFB1 repression by qRT-PCR. Heat-inactivated encapsulated bacteria showed no repression of CXCL8 and DEFB1, i.e., no difference (p > 0.05) compared to viable encapsulated S. pneumoniae (Dead Encap.). While infection with heat-inactivated ∆cps2E repressed CXCL8 to the level of viable ∆cps2E, DEFB1 was more repressed (p < 0.05) by dead ∆cps2E than by viable unencapsulated pneumococci

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