Fig. 7
From: Topoisomerase II beta interacts with cohesin and CTCF at topological domain borders
DNA supercoiling around CTCF binding sites. a Comparison of DNA supercoiling around CTCF binding sites in untreated human retinal pigment epithelial cells (RPE-1), after 5 h inhibition of RNA polymerase with alpha-amanitin and after 2 h of recovery from inhibition. b Changes in DNA supercoiling after 5 h treatment with TOP2 inhibitor ICRF-193 or TOP1 inhibitor camptothecin compared to untreated RPE-1 cells. c DNA supercoiling around CTCF sites after simultaneous inhibition of transcription and topoisomerases in RPE-1 cells. Direction of CTCF motif is indicated by the arrow. The signal on randomly generated genomic intervals is shown as dashed lines and compared with the observed signal (solid lines), all p values are <10–16 (Kolmogorov–Smirnov test). Data and methods used to generate this original analysis were previously published by Naughton et al. [65]