Fig. 1

TOP2B interactome. a Overview of BioID method [31, 32]. In BioID, mutated Escherichia coli biotin protein ligase BirA* is fused with the protein of interest (bait) resulting in vivo biotinylation of proximal and interacting proteins of the bait protein. Subsequent to cell lysis, biotinylated proteins are captured by streptavidin and identified by mass spectrometry. b High-confidence interaction partners of TOP2B (n = 25, FDR ≤5 %) are visualized as a network displaying the average spectral counts of a given prey protein. Known TOP2B interacting proteins are shown in bold. Cohesin complex proteins (RAD21, NIPBL, PDS5A, PDS5B, STAG1, STAG2, SMC1A) and CTCF are indicated. c Enrichment Map network visualization of statistically over-represented biological processes in the TOP2B interactome (FDR q <0.05). Nodes in the network represent enriched processes and pathways that are grouped according to prevalent functional themes. Node size is proportional to the total number of genes within each gene set. Genes corresponding to interacting proteins are shown