Table 1 Mutations of genes in the genome of zebrafish induced by SGN
Target | gDNAs-R | Clone number | Deletion (bp) | Insertion (bp) | Guide DNA spacer (bp) |
|---|---|---|---|---|---|
GFP reporter gene in Tg(flk1:eGFP) zebrafish | gDNA-gfp-R0 | #1-44 | 659 | 3 | 0 |
#1-46 | 659 | 3 | 0 | ||
gDNA-gfp-R8 | / | / | / | 8 | |
gDNA-gfp-R18 | #3-18 | 651 | 1 | 18 | |
#3-21 | 651 | 1 | 18 | ||
#3-23 | 659 | 3 | 18 | ||
gDNA-gfp-R32 | #4-2 | 651 | 1 | 32 | |
#4-3 | 651 | 1 | 32 | ||
#4-4 | 651 | 1 | 32 | ||
#4-9 | 639 | 17 | 32 | ||
#4-11 | 649 | 20 | 32 | ||
#4-14 | 659 | 3 | 32 | ||
#4-16 | 644 | 0 | 32 | ||
#4-17 | 648 | 1 | 32 | ||
#4-18 | 659 | 3 | 32 | ||
#4-19 | 644 | 0 | 32 | ||
#4-22 | 649 | 20 | 32 | ||
#4-33 | 649 | 20 | 32 | ||
#4-34 | 644 | 0 | 32 | ||
#4-37 | 651 | 1 | 32 | ||
#4-39 | 639 | 17 | 32 | ||
#4-42 | 648 | 1 | 32 | ||
#4-44 | 639 | 17 | 32 | ||
#4-48 | 651 | 1 | , | ||
gDNA-gfp-R50 | #5-9 | 651 | 0 | 50 | |
#5-11 | 651 | 0 | 50 | ||
#5-14 | 651 | 0 | 50 | ||
#5-15 | 596 | 12 | 50 | ||
#5-20 | 651 | 0 | 50 | ||
#5-23 | 651 | 0 | 50 | ||
#5-24 | 651 | 0 | 50 | ||
#5-27 | 651 | 0 | 50 | ||
#5-28 | 651 | 0 | 50 | ||
#5-29 | 611 | 3 | 50 | ||
#5-31 | 651 | 0 | 50 | ||
#5-48 | 611 | 3 | 50 | ||
znf703 endogenous gene | gDNA-znf703-R50 | #6 | 754 + 11 | 0 | 50 |
cyp26b1 endogenous gene | gDNA-cyp26b1-R32 | #7-1 | 2610 | 0 | 32 |
#7-2 | 2610 | 0 | 32 | ||
#7-3 | 2610 | 0 | 32 |