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Fig. 8 | Genome Biology

Fig. 8

From: An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease

Fig. 8

The proposed mechanism underlying large deletions by SGN. One gDNA hybridizes to a single strand of zebrafish genomic DNA to form the 3′ flap-structure. SGN binds to the 3′ flap-structure and cleaves the single strand of zebrafish genomic DNA to create a nicked structure that can be recognized by SGN. One SGN molecule binds to the nicked structure and cleaves the single strand of zebrafish genomic DNA to produce a new nicked structure. This means that once the cleavage starts, it repeats successively; and finally the disrupted genomic DNA is repaired by the DNA repair pathway in vivo

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Genome Biology

ISSN: 1474-760X

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