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Fig. 3 | Genome Biology

Fig. 3

From: An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease

Fig. 3

The effect of different unpaired 3′ nucleotides on the DNA cleavage created by SGN. Schematic (top) shows the unpaired 3′ nucleotide of gDNA on the ssDNA target. The red arrows indicate the possible cleavage sites according to the sizes of the cleaved products. The denatured-PAGE results (below) show the clearly cleaved products by SGN without obvious difference among the efficiency of the different unpaired types. (a) S-1 ssDNA target with gDNA-1, gDNA-1-G, and gDNA-1-T; (b) S-3 ssDNA target with gDNA-3, gDNA-3-G, and gDNA-3-A; (c) S-4 ssDNA target with gDNA-4, gDNA-4-A, and gDNA-4-T; and (d) S-5 ssDNA target with gDNA-5, gDNA-5-T, and gDNA-5-G

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