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Fig. 6 | Genome Biology

Fig. 6

From: Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm

Fig. 6

Snapshot and temporal scRNA-seq profiling on progenitor cell states. scRNA-seq from snapshots of lineage-specific progenitors revealed that hypoxia enhances DE differentiation in a time-sensitive manner, likely acting on the mesendodermal progenitors. The differentiation trajectory was reconstructed by Wave-Crest (blue arrow) using time course scRNA-seq along the differentiation towards DE cells. Results from loss-of-function and gain-of-function experiments demonstrated that KLF8 function as a positive regulator mediating cell states transitions from T + mesendoderm to CXCR4 + DE cells. We hypothesize that KLF8 can suppress T + state and in turn enhance the activation of CXCR4 + state. The molecular mechanisms of how KLF8 directly or indirectly suppress T + state require future investigation

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