Fig. 1

Snapshot scRNA-seq analysis of human ES-derived progenitors. a Schematics of experimental strategy. Human ES-derived lineage-specific progenitors were profiled at the single-cell resolution. b Principal component analysis (PCA) of all the cell types profiled. Shown are PC4 vs. PC1 and PC2 vs. PC4. c Hierarchical clustering analysis of progenitors differentiated from H1 cells with selected lineage-specific markers shown on the right. d PCA of all the cell types profiled, shown are PC5 vs. PC6. The light green shade highlights all the single DE cells. e Enrichment analysis of PC5 shown in (d). Bar graph shows the significant z scores of selected GO terms. Summary on collections of GO terms are shown on the right. Dashed line indicates statistical significant threshold at z score = 1.62 (one tailed p value <0.05). NPC neuronal progenitor cell, DEC definitive endoderm cell, EC endothelial cell, TB trophoblast-like cell, HFF human foreskin fibroblasts