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Fig. 6 | Genome Biology

Fig. 6

From: Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability

Fig. 6

Elevated H4K20me3 levels reinforce a stable proliferative arrest in senescent cells. a Western blot of indicated proteins from whole cell extracts of IMR90 cells 12 days after infection with vector control (CON), Myc-tagged SUV420H1 (H1), Myc-tagged SUV420H2 (H2) or H-RASG12V (OIS). b Western blot of histone H4 and indicated H4K20 modifications from whole cell extracts described in a, normalized for total histone H4 content. c Immunofluorescent images of Myc-tagged SUV420H1 or SUV420H2 and H4K20me3 in CON, H1, and H2 IMR90 cells from a. d Growth curves expressed as cumulative population doublings for CON, H1, and H2 IMR90 cells measured for 38 days after infection. e Western blot of indicated proteins from whole cell extracts of CON, H1, and H2 IMR90 cells from d 3 and 38 days after infection. f Western blot of indicated proteins from whole cell extracts of CON and H2 IMR90 cells collected 5, 10, or 15 days after infection with either vector control (CON) or H-RASG12V (OIS); (s) and (l) denote short and long autoradiographic exposures, respectively

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Genome Biology

ISSN: 1474-760X

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