The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum

Table 1 Candidate mRNA-binding proteins captured with high confidence in trophozoite- and schizont-stage parasites

Locus	Description	Average spectra detected	Fold enrichment^a				Pfam RNA-binding domain	QSPEC p value^b	Chi-square p value^c
Locus	Description	Average spectra detected	Troph. 1	Troph. 2	Schizont 1	Schizont 2	Pfam RNA-binding domain	QSPEC p value^b	Chi-square p value^c
PF3D7_1006800	RBP, putative	284	10.3	23.7	13.2	6.2	RRM	0	8.6E-144
PF3D7_1409800	PfCELF2 (formerly Bruno)	237	4.7	7.4	8.1	6.0	RRM, RNA_bind	0	2.3E-126
PF3D7_0916700	Musashi (HoMu)	187	11.4	5.5	37.0	4.0	RRM	0	1.7E-95
PF3D7_1359400	PfCELF1	176	4.8	11.7	8.5	9.9	RRM, RNA_bind	0	2.3E-85
PF3D7_0929200	RBP, putative	179	6.7	3.5	5.7	1.4	RRM	0	2.8E-74
PF3D7_1236100	PfCARP	151	6.9	1.9	13.0	2.8	RRM, RNA_bind	0	5.4E-52
PF3D7_0105200	RAP protein, putative	53	2.8	2.8	24.5	13.2	RAP	0	7.9E-26
PF3D7_1346300	PfAlba2	92	30.7	2.6	63.7	53.0	Alba	0	8.6E-26
PF3D7_1021900	Conserved protein	113	2.1	2.0	2.6	3.9	RNA_GG_bind	0	9.7E-25
PF3D7_0823200	RBP, putative	112	1.8	1.9	5.4	1.5	RRM, RNA_bind, DUF1866	0	2.0E-24
PF3D7_1105800	Conserved protein	53	4.0	0.6	5.6	13.2	-	0	8.8E-23
PF3D7_1006200	PfAlba3	131	4.4	5.1	12.8	11.6	Alba	0	2.8E-18
PF3D7_0703500	Erythrocyte membrane-associated antigen	80	0.9	2.9	5.7	4.4	DEAD	0	4.4E-18
PF3D7_0629400	RBP, putative	36	Infinity	7.0	Infinity	0	RRM	0	1.1E-14
PF3D7_1107300	PfPAIP1	73	1.2	3.2	1.9	4.4	-	0	1.2E-13
PF3D7_1474900	CITH	31	3.1	6.0	4.8	13.2	LSM14, FDF, SM_ATX	0	4.0E-13
PF3D7_1118200	Heat shock protein 90	41	3.1	4.9	8.8	3.6	-	0	3.4E-09
PF3D7_0810600	RNA helicase, putative	52	3.6	2.9	4.2	0	DEAD	0	1.5E-07
PF3D7_1347500	PfAlba4	103	4.5	4.7	6.3	7.4	-	0	2.1E-06
PF3D7_1330800	Conserved protein	37	8.4	1.8	Infinity	0	RRM	0	6.8E-04

^aEnrichment of proteins in mRNA-interactome capture sample as compared to the corresponding RNase-treated control sample
^bAnalysis of differential protein abundance in the capture versus control data using the combined spectral counts from all four experiments. Reported p values are FDR-corrected (5 % [80])
^cAnalysis of protein abundance in the capture data compared to an existing P. falciparum mass spectrometry data set [46] to assess the likelihood of contamination. Reported p values are FDR-corrected using the Benjamini-Hochberg method (5 %)
Troph. trophozoite

ISSN: 1474-760X