Fig. 3

Cross-study analysis of MIT specificity scores as calculated by CRISPOR versus the number and strength of off-targets found. a For 31 guide sequences, CRISPOR guide specificity scores are shown (x-axis), as well as the number of off-targets (y-axis) and sum of off-target modification frequencies (circle size). The guide with a specificity score of 88 has no single detected off-target. b The specificity scores of 31 tested guide sequences (blue) versus the specificity scores of all unique guides (unique 20mers followed by NGG) in human coding regions (green). Specificity scores were calculated using the CRISPOR website. For a version of this figure with specificity scores calculated by the MIT site, see Additional file 9: Figure S4