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Fig. 5 | Genome Biology

Fig. 5

From: Ligation-free ribosome profiling of cell type-specific translation in the brain

Fig. 5

mTOR controls TOP motif-containing genes in the brain. Camk2a-RiboTag mice were treated for 1 h with the ATP-competitive mTOR inhibitor AZD-8055 and were used to generate ligation-free ribosome profiling libraries from brain tissue and fluorescence imaging data. a Treatment for 1 h with AZD-8055 was sufficient to drastically decrease levels of phosphorylated Rps6 in mouse brains. HA-staining indicates the presence of HA-tagged Rpl22 (RiboTag) in cells expressing Camk2a. b Comparison of RNA and TE fold changes between AZD-8055-treated and untreated mice. TE exhibits larger amplitude changes than RNA levels in response to mTOR inhibition in the brain. The TE of TOP motif-containing genes are greatly reduced. c We used RiboDiff to identify genes with significant differential translation efficiency and DESeq2 to identify genes with significant differential RNA expression in treated versus untreated mice. The Venn diagram shows the overlap between genes with significant translational reduction after AZD-8055 treatment, ribosomal proteins, and TOP motif-containing genes

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