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Fig. 4 | Genome Biology

Fig. 4

From: Ligation-free ribosome profiling of cell type-specific translation in the brain

Fig. 4

Features of 5′ UTRs are associated with CDS translation. a The percentage of cell type-specific genes with at least one ribosome footprint mapping to their 5′ UTR is plotted together with the percentage of cell type-specific genes with 5′ UTR ribosomal density and also containing a uAUG sequence. These values are highly consistent across cell types. b Genes containing a uAUG and 5′ UTR ribosomal density had lower CDS TE compared with genes without a uAUG. This effect was consistent across multiple cell types and was significant for myelinating, microglial, and endothelial cells. Furthermore, this effect was seen regardless of cell-type specificity. c Heatmap showing the relationship between 5′ UTR GC content, 5′ UTR length and CDS TE. Very high and very low GC content are associated with lower median TE. As the length of the 5′ UTR increases, the median TE of the CDS decreases. d, e The relationships between GC content (d) and 5′ UTR length (e) are independently plotted against median TE for each length or GC-content bin; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001

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