Fig. 1

Comparison of ligation-free ribosome profiling with conventional methods. a The steps involved in conventional ribosome profiling and ligation-free ribosome profiling. b The power spectrum of 5′ mapping positions from coding sequence (CDS) reads resulting from the ligation-free ribosome profiling method shows clear three-base periodicity that is characteristic of ribosome profiling libraries and reflects the single-codon translocation of the ribosome. c Gene body distribution of mapped reads from ligation-free ribosome profiling show strong preference for CDS, an additional property inherent to ribosome profiling libraries. d Comparison of the number of uniquely mapped reads per gene in libraries generated with footprints from mouse forebrains prepared with a conventional ribosome profiling strategy and the ligation-free method; the Pearson correlation r = 0.97 indicates a concordance between the two methods. e Saturation analysis showing the number of unique genes detected following downsampling of ligation-free ribosome profiling and conventional ribosome profiling. f Saturation analysis showing the number of unique footprints detected following downsampling of ligation-free ribosome profiling and conventional ribosome profiling. PAGE polyacrylamide gel electrophoresis, PCR polymerase chain reaction, ssRNA single-stranded RNA, UTR untranslated region