Fig. 5

Retrozymes in the genome of the eucalyptus (Eucalyptus camaldulensis). a Northern blot analysis of RNAs (~20 μg) obtained from E. camaldulensis leaves, sprouts and flowers carried out as described in Fig. 3. 0.1 ng of a (+)RNA transcript of a genomic retrozyme from E. camaldulensis was included. The positions of the linear and circular RNAs are indicated. Ethidium bromide staining of the 7.5S rRNA band is shown at the bottom as a loading control. b Minimum free energy secondary structure prediction for a retrozyme RNA of 979 nt derived from a genomic retrozyme of E. camaldulensis (entry BADO01091291.1). Numbering and colors are shown as in Fig. 3