Table 1 mRNA and circRNA quantification by ERCC/RGC-A80 normalization algorithm
From: Tracing the expression of circular RNAs in human pre-implantation embryos
Stage | Oocyte | Zygote | 2-cell | 4-cell | 8-cell | Morula | Early blastocyst | Blastocyst | Hatched blastocyst |
|---|---|---|---|---|---|---|---|---|---|
Average mRNA copy number (106) | 66.4 | 29.0 | 24.6 | 17.0 | 15.7 | 38.1 | 45.9 | 104.5 | 150.7 |
Average circRNA copy number | 56,059 | 48,983 | 80,410 | 190,008 | 148,656 | 149,508 | 93,745 | 40,194 | 28,774 |
circRNA/hosting gene (median) | 9 % | 7 % | 9 % | 12 % | 14 % | 25 % | 22 % | 17 % | 20 % |
No. of circRNA genes per embryo | 149 | 299 | 358 | 727 | 602 | 376 | 519 | 252 | 80 |
No. of circRNA transcript types per embryo | 179 | 392 | 488 | 1,115 | 869 | 509 | 630 | 278 | 85 |
No. of circRNA reads per embryo | 585 | 1529 | 2248 | 8059 | 8024 | 2702 | 2911 | 838 | 225 |