Fig. 6

Expression pattern of de novo assembled transcripts during human pre-implantation development. a The box plots show that the expression levels of the novel assembled genes are lower than those of the RefSeq genes but higher than those of the lncRNAs from Noncode V4.0 and our previous paper. b Hierarchical clustering analysis of novel genes indicating stage-specific expression patterns during human pre-implantation. Based on the heat map, these novel genes can be divided into three major types: early zygotic genes, maternal genes, and late zygotic genes. c The pattern of total expression levels of all novel 2322 candidate genes revealing that genes enriched before the 8-cell stage are mostly maternal genes