Fig. 2

mRNA quantification and analysis of differential expressed genes. a mRNA copy numbers in each individual embryo during human pre-implantation development were estimated by the algorithm of ERCC/RGC-A80 normalization. The calculation process is described in the section of “Methods.” b Clusters of differential expressed genes by normalized RPKM accounting the total mRNA content in each sample during pre-implantation development. The first cluster includes 5573 maternal genes, such as ZP family genes, TET3, and GDF9. These 7427 genes from the 2–5 clusters which are activated from the 4-cell stage during the subsequent developmental stages are defined as zygotic genes. The representative GO terms of each cluster and corresponding P value are shown at the right panel