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Fig. 2 | Genome Biology

Fig. 2

From: The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the basis of parasitism and virulence

Fig. 2

Example of differential gene expression clusters in the context of nematode biology. The transcriptome of G. rostochiensis was sequenced in duplicate for four key stages across the life cycle: dormant cysts; hydrated eggs; hatched infective juveniles (J2); and feeding 14 days post infection (dpi) females. A subset of the 6720 genes differentially expressed (FDR <0.001, min fold 4) are grouped into expression clusters which describe the genes specifically upregulated at various life stages. Clusters which uniquely describe each life stage (1st order), describe two life stages (2nd order) or describe three of the four life stages (3rd order) are identified. Further, some expression clusters show a stepwise increase (or decrease) in expression as the nematode transitions through its life cycle (trans). For all expression clusters, mean centred log fold-change of expression is plotted for each of two biological replicates for each life stage in the following order: Cyst, egg, J2, 14 dpi female. All genes in each cluster are drawn with grey bars, the average of which is shown in blue

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