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Fig. 2 | Genome Biology

Fig. 2

From: Single-cell analysis of CD4+ T-cell differentiation reveals three major cell states and progressive acceleration of proliferation

Fig. 2

Deep transcriptomic analysis reveals three discrete cell states during Th2 differentiation. a Heatmap of all ~14,000 protein-coding genes (rows) per generation (columns). At the top and bottom, genes with a monotonic increase/decrease are shown. In the middle, genes are ranked according to distance from G4P and G0N. b Number and percentage of differentially expressed genes between samples. c Gene Ontology enrichment analysis was performed on differentially expressed genes between G0N and G2N (gray bars) and G4N and G4P (green bars). The threshold p value of 0.05 is shown as a dotted line. d Average Z-scores for upregulated genes belonging to different functional categories calculated from heatmaps in Additional file 4: Figure S2d. e Heatmap of Th2 signature gene expression across generations. f A three-state differentiation model in which G0N cells are named A cells (“Activated” cells), G2N and G4N cells are named B cells (“Proliferating” cells) and G4P cells are named C cells (“Citokine-producing” cells)

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