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Fig. 8 | Genome Biology

Fig. 8

From: Genomic screens identify a new phytobacterial microbe-associated molecular pattern and the cognate Arabidopsis receptor-like kinase that mediates its immune elicitation

Fig. 8

XPS1 binds specifically to xup25. a Coomassie stain of purified XPS1 ectodomain used in MST assays. b Quantification of binding between the XPS1 ectodomain and xup25 by label-free MST. The XPS1 ectodomain was kept at a constant concentration (0.5 μM) whereas varying peptide concentrations were added. Data points indicate the difference in normalized fluorescence (%) generated by xup25 binding to the XPS1 ectodomain. Curves are plots of xup25 concentrations against percent changes of normalized fluorescence (ΔFnorm [%] y-axis). Curve fitting was performed by using the NT affinity analysis software from Nanotemper. The EC50 value calculated at MST power of 60 % is indicated on top. The binding profile is representative of two independent assays performed with two independent protein preparations (see Additional file 9: Figure S8). The standard error of the regression fit is 2.866732

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Genome Biology

ISSN: 1474-760X

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