Fig. 6

xup25-induced callose deposition is XPS1-dependent. Leaves from A. thaliana ecotype Col-0 or xps1-1 plants were pressure infiltrated with water or 10 μM of peptide. After 24 h of treatment the leaves were harvested, cleared, and callose deposits were stained prior to epifluorescent microscopy. a The proportion of the image with callose present was determined and data from a single representative trial is shown (n = 6, *P <0.05, ***P <0.001, pairwise Student’s t-test, corrected with Holm-Bonferroni). b Representative images of the callose deposits for each treatment are shown. Callose deposits are indicated by dark spots; the images have been converted to gray scale and inverted. The experiment was repeated four times in total with similar results, for a total n = 26