Fig. 3

Simultaneous profiling of promoter methylation and gene expression from a single neuron. a Representative scatter plot for CGI promoter methylation level and transcription level of genes at whole genome wide within a representative single cell. Promoter methylation level was calculated by the ratio of methylated CpG sites over all CpG sites within the promoter region. Expression level was transformed to expression percentile. b Representative scatter plot for non-CGI promoter methylation level and transcription level of individual genes within a representative single cell. c Dot plot of Pearson correlation coefficients between transcription level (as expression percentile) and promoter methylation. d Representative example of genes with hypomethylation promoter and dynamic expression. Each point represents a single cell. e Representative example of genes that differential promoter methylation is negatively correlated with gene expression. Each point represents a single cell. f Representative example of genes with hypermethylation promoter and high expression. Each point represents a single cell. g Genome browser tracks for Slc25a5 showing promoter hypermethylation and high gene expression in three representative male single cells. Red bars indicate the methylation CpG sites and blue bars indicate the unmethylated CpG sites. RNA transcription level is shown in green. The CpG island reference and MspI cut sites are in dark green and purple, respectively. *p <0.0001