Fig. 1

Single-cell cytosol transcriptome resembles single-soma transcriptome. a Schematic of the single-cell transcriptome and methylome sequencing (scMT-seq) method. b Comparison of single-cell cytosol RNA-seq and soma RNA-seq in terms of the coverage of gene number. Only genes with reads per kilobase per million (RPKM) >0.1 were counted. c Scatter plot of transcript expression levels in cytosol (x-axis) or soma (y-axis) samples. Red dots indicate the significantly differentially expressed genes (p <0.01) and gray dots indicate genes that are not differentially expressed. d Principal component analysis for DRG single soma and cytosol RNA-seq libraries. The relative expression levels of known marker genes for specific subgroups are shown in color. Red represents high expression while blue represents low expression. Solid circles represent cytosol; empty squares represent soma