Fig. 1

Flowchart of a typical single cell omic sequencing project. A typical single-cell sequencing project comprises four major steps: experimental design, performing the experiments, bioinformatic analyses and verification of the discoveries. Here we use a single-cell RNA-seq project as an example. Note that if the project fails at any step, researchers should go back to previous steps to identify the cause of the failure and re-design accordingly. In a real project, this process may need to be repeated several times. FACS fluorescence-activated cell sorting, MACS magnetic-activated cell sorting, qPCR quantitative polymerase chain reaction