Fig. 7

Pseudotime analysis with Waterfall predicts a novel, non-neural X2 lineage. a PCA plot for the X1-containing groups. b PCA plot for the X2-containing groups. c PCA plot for the predicted X2 lineage. d Plot of X2 lineage (Groups 1, 2, 4, 5, and 6) cells ordered along pseudotime. Red line, linearized MST connecting k-mean centers; grey arrows, direction of lineage progression; y-axis, distance of cell to its nearest k-means center. e–g Expression profiles of previously described genes in this potential X2 lineage: e stem cell markers piwi-1 and vasa-1; f differentiation regulator mex3-1; g pan-neural marker pc-2. h Expression patterns of candidate markers for this predicted X2 lineage. Red line, local polynomial regression fit; shaded region, 95 % confidence interval