Fig. 4

In situ identification of piwi-2 ⁺ piwi-1 ^lo stem cells. a Colorimetric WISH of piwi-1. b Colorimetric WISH of piwi-2. Scale bars = 100 μm. c High magnification images of the stem cell regions in the head between the brain lobes (top row) and tail stripe (bottom row) are shown. Arrowheads, piwi-2 ⁺ piwi-1 ^-PIWI-1⁺ cells. d Quantification of piwi-2 and piwi-1 colocalization in the stem cell areas in the head and tail; n = 5, p = 0.00035. e Quantification of piwi-2 and PIWI-1 colocalization in the stem cell areas in the head and tail; n = 5, p = 0.89742. Regions included in counts are outlined by a dashed line in c