Fig. 2

Neural gene sets are enriched in some groups compared with bulk X1 data. a–c Gene set enrichment analysis (GSEA) results for Group 3 (FDR < 0.01, p < 0.001) (a), Group 1 (FDR < 0.05, p < 0.001) (b), and Group 2 (FDR < 0.05, p < 0.001) (c). Nodes represent gene sets and node size is proportional to the GSEA nominal enrichment score. Node color represents the group in which the gene set is enriched. The width of the green edges represents the number of genes shared between the connected nodes. Similar gene sets are circled and their function is labeled. Group gene expression profiles are averages of the single-cell expression profiles in the group. d Heatmap displaying log2 normalized counts of known markers for neural, stem cell, sigma class, zeta class, and non-neural tissue. Columns are single cells from the group noted above the heatmap. GPCR G protein-coupled receptor