Fig. 3
From: Beyond comparisons of means: understanding changes in gene expression at the single-cell level
Summary of changes in expression patterns (mean and over-dispersion) for SCs vs P&S samples (EFDR = 5 %). a Volcano plots showing posterior medians of LFCs against estimated tail posterior probabilities. Left panels relate to the test where we assess if the absolute LFC in overall expression between SCs and P&S samples exceeds a minimum threshold τ 0. Estimates for LFCs in overall expression are truncated to the range (−1.5,1.5). Pink and green dots represent genes highlighted to have higher overall expression in the SC and P&S samples, respectively. Right panels relate to the test where we assess if the absolute LFC in biological over-dispersion between SC and P&S samples exceeds a minimum threshold ω 0. In all cases, horizontal dashed lines are located at probability cut-offs defined by EFDR = 5 %. Pink and green dots represent genes highlighted to have higher biological over-dispersion in the SC and P&S samples, respectively. b Bins in the horizontal axis summarize changes in overall expression between the groups. We use SC+ and P&S+ to denote that higher overall expression was detected in SC and P&S samples, respectively [the central group of bars (No diff.) corresponds to those genes where no significant differences were found]. Colored bars within each group summarize changes in biological over-dispersion between the groups. We use pink and green bars to denote higher biological over-dispersion in SC and P&S+ samples, respectively (and gray to denote no significant differences were found). The numbers of genes are displayed in log-scale. LFC log-fold change, P&S pool-and-split, SC single cell