Fig. 9

Loss of TCTP and ECHIDNA functions also impairs post-fertilization events. a Reciprocal test crosses of the tctp-1 mutation showing low penetrance male-specific phenotypic induction in F1 siliques as well as significantly reduced male transmission efficiency scored by PCR genotyping as well as GUS-staining of LAT52-GUS-tagged T-DNA insertion. The micrograph on the far right shows seedling segregation of self-fertilized +/​tctp-1 plants, supporting the near complete block of tctp-1 allele transmission through the male. b Dissected siliques of wild-type and self-fertilized +/​tctp-1 plants. Asterisks indicate wild-type-like seeds, black arrowheads indicate underdeveloped chlorotic mutant seeds, red arrowheads and yellow arrows show arrested embryos with failed embryogenesis soon after fertilization as well as unfertilized ovules. c Frequency and positioning of mutant seeds within dissected siliques. The random distribution of mutant seeds within +/​tctp-1 siliques suggests tctp-1 mutant pollen tube growth is competent. d Frequency of tctp-1 mutant seed phenotypic classes observed (n = 25 siliques per line). Error bars represent standard deviation (stdv). e Dissected siliques of self-fertilized ech-/- plants showing the high frequency of failed fertilization, defective embryogenesis, as well as “wild-type-like” seeds (asterisks). f Frequency and random positioning of ech mutant embryos/unfertilized ovules within ech-/- self-fertilized siliques implying ech pollen tubes can grow to the base of the pistil and target ovules for fertilization. g Frequency of mutant seed phenotypic classes observed (n = 25 siliques per line). Error bars represent standard deviation (stdv). h Embryogenesis lethality in ECHIDNA-deficient embryos showing stages and frequency of embryo arrest. Scale bars = 10 μM. gn germ cell nuclei, na not applicable, sn sperm cell nuclei, vn vegetative cell nuclei, wt wild type