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Fig. 7 | Genome Biology

Fig. 7

From: Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization

Fig. 7

LLG3 secretion is compromised in yip4a-1;yip4b mutant pollen and pollen tubes. a Subcellular localization of LLG3-mRFP under native promoter showing cytosolic foci-like aggregates. vn vegetative cell nucleus, sn sperm cell nuclei. b Top: in the yip4a-1;yip4b mutant, the majority of the pollen grains displayed distinct cytosolic aggregates different from those observed in the wild type. Bottom: z-stack projection of 25 confocal slices showing distinct vegetative cell “bird cage-like” localization that was not observed in wild-type pollen grains. c Localization of LLG3-mRFP in wild-type pollen tubes grown in vitro for 16 h showing localization in likely secretory vesicles of uniform size and partially in the pollen tube ER (top three panels). In contrast, yip4a-1;yip4b mutant pollen tubes displayed a significantly higher frequency of LLG3-mRFP-marked endomembrane aggregated vesicles of variable sizes (bottom three panels). However, the ER localization was not greatly affected. d LLG3-mRFP also specifically accumulated at the subapical domain (top panel, arrow). This accumulation was also not significantly affected in yip4a-1;yip4b mutant pollen tubes (bottom panel). e In tobacco pollen tubes, similar localization in secretory vesicles was also observed (top panel, arrows) and occasionally LLG3-mRFP as well as LLG3-sGFP showed pollen tube tip-specific localization (bottom panels, arrowheads). f Absence of LLG3-mRFP protein in Arabidopsis unfertilized ovules and embryos soon after fertilization (18 h after pollination). Rarely, LLG3-mRFP could be detected at the embryo proper (EP) zone in some fertilized ovules. MC micropylar, EP endosperm proper, CE chalaza end endosperm. Scale bars for a and b = 30 μM and for c–e = 10 μM

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