Skip to main content
Fig. 2 | Genome Biology

Fig. 2

From: Single-CpG resolution mapping of 5-hydroxymethylcytosine by chemical labeling and exonuclease digestion identifies evolutionarily unconserved CpGs as TET targets

Fig. 2

Validation of the SCL-exo strategy for single-CpG resolution mapping of 5hmC in genomic DNA from P19 cell-derived NPLCs. a Average CpG density along read length from SCL-seq (375,104 reads from chr7) and from SCL-exo (899,652 reads from chr7). b IGB visualization of hMeDIP and SCL-exo signals from three technical replicates in the 3’ region of the Centg2 gene. c Genome-wide correlation coefficient value (Pearson’s coefficient, r) for.wig files corresponding to two technical replicates of SCL-exo identified (id) CpGs. d Genome-wide correlation coefficient value for one replicate of hMeDIP and one replicate of SCL-exo. e Venn diagram indicating the percentage of SCL-exo id CpGs (called from the consensus.wig file with a coverage threshold of 20×) included in hMeDIP-seq peaks (called with a threshold (th) of 12×). f Genome-wide correlation coefficient value for one replicate of hMeDIP and one replicate of SCL-seq. g Genome-wide correlation coefficient value for one replicate of SCL-exo and one replicate of SCL-seq. h Correlation between SCL-exo signal at id CpGs (number of reads) and their percentage of hydroxymethylation determined with the EpiMark kit for 27 selected CCGG sites (r: Pearson’s correlation coefficient)

Back to article page

Genome Biology

ISSN: 1474-760X

Contact us