Depletion of Abundant Sequences by Hybridization (DASH): using Cas9 to remove unwanted high-abundance species in sequencing libraries and molecular counting applications

Table 1 Summary of depletion/enrichment results in DASH-treated clinical CSF samples

Pathogen	Read count (percentage duplicates)		Targeted genes (fpkm)				Representative pathogenic gene^a (fold change)		R² non-targeted genes, untreated versus DASHed
	Read count (percentage duplicates)		12S		16S		Representative pathogenic gene^a (fold change)
	Un-treated	DASHed	Un-treated	DASHed	Un-treated	DASHed	Un-treated	DASHed
B. mandrillaris	1.81 M (26 %)	2.54 M (15 %)	298,922	28,005	380,073	93,164	0.028 %	0.102 %	0.992
B. mandrillaris	1.81 M (26 %)	2.54 M (15 %)	298,922	28,005	380,073	93,164	(3.6×)		0.992
C. neoformans	2.95 M (27 %)	3.43 M (11 %)	361,501	37,168	342,857	93,703	1.5 %	15.4 %	0.986
C. neoformans	2.95 M (27 %)	3.43 M (11 %)	361,501	37,168	342,857	93,703	(10.3×)		0.986
T. solium	2.38 M (33 %)	1.89 M (30 %)	451,044	46,993	317,640	43,257	12.0 %	44.3 %	0.994
T. solium	2.38 M (33 %)	1.89 M (30 %)	451,044	46,993	317,640	43,257	(3.7×)		0.994

^a Representative genes are 16S for Balamuthia mandrillaris and 18S for Cryptococcus neoformans and Taenia solium

ISSN: 1474-760X