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Table 1 Summary of depletion/enrichment results in DASH-treated clinical CSF samples

From: Depletion of Abundant Sequences by Hybridization (DASH): using Cas9 to remove unwanted high-abundance species in sequencing libraries and molecular counting applications

Pathogen Read count (percentage duplicates) Targeted genes (fpkm) Representative pathogenic genea (fold change) R2 non-targeted genes, untreated versus DASHed
12S 16S
Un-treated DASHed Un-treated DASHed Un-treated DASHed Un-treated DASHed
B. mandrillaris 1.81 M (26 %) 2.54 M (15 %) 298,922 28,005 380,073 93,164 0.028 % 0.102 % 0.992
(3.6×)
C. neoformans 2.95 M (27 %) 3.43 M (11 %) 361,501 37,168 342,857 93,703 1.5 % 15.4 % 0.986
(10.3×)
T. solium 2.38 M (33 %) 1.89 M (30 %) 451,044 46,993 317,640 43,257 12.0 % 44.3 % 0.994
(3.7×)
  1. a Representative genes are 16S for Balamuthia mandrillaris and 18S for Cryptococcus neoformans and Taenia solium