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Fig. 3 | Genome Biology

Fig. 3

From: Depletion of Abundant Sequences by Hybridization (DASH): using Cas9 to remove unwanted high-abundance species in sequencing libraries and molecular counting applications

Fig. 3

Normalized coverage plots of DASH-treated (orange) and untreated (blue) libraries generated from patient cerebrospinal fluid (CSF) samples with confirmed infections. Targeted mitochondrial rRNA genes (left) and representative genes for pathogen diagnosis (right) are depicted for the following: patient 1, Balamuthia mandrillaris (a), patient 2, Cryptococcus neoformans (b), patient 3, Taenia solium (c). Across all cases, the DASH technique significantly reduced the coverage of human 12S and 16S genes by an average of 7.5-fold while increasing the coverage depth for pathogenic sequences by an average 5.9-fold. See Table 1 for relevant data

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Genome Biology

ISSN: 1474-760X

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