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Fig. 4 | Genome Biology

Fig. 4

From: Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design

Fig. 4

Quantitative comparison of full-length and truncated gRNAs. a gRNA effects (see methods) of 182 full-length gRNAs (20 nt of complementarity to the target) are plotted on the y-axis, and their truncated counterparts (18 nt of complementarity) on the x-axis. In all cases, gRNA-expressing strains were grown in the appropriate reference small molecule. Dotted and solid gray lines demarcate gRNA effects of 0 and -2, respectively. b Heatmaps illustrating growth defects induced by gRNAs containing different mismatches to the target sequence. Full-length and truncated gRNAs are arranged by target gene on the y-axis. The reference small molecule is labeled on the right. The mismatch position of each gRNA relative to the PAM is indicated on the x-axis (gRNAs matching the target sequence perfectly are on the far left). Missing values are indicated with an X. c As in (a), only the mismatched gRNAs described in (b) are plotted. Points are color-coded based on target gene (see legend). Large points represent the ‘perfect’ gRNAs, all other points represent gRNAs containing mismatches.

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