Fig. 3

Erg25 regulates fluconazole sensitivity. a Strains containing gRNA constructs directed against ERG3, ERG11, ERG25, and the empty vector control (indicated above each panel), were grown in 63.1 μM fluconazole, in either the presence (blue), or absence of 250 ng/μL ATc (red). In each panel, optical density (OD) is plotted on the y-axis, as a function of time on the x-axis. Loss of ERG3 function was previously shown to confer fluconazole resistance [55, 56] and served as a positive control. b Similar to (a). The parental BY4741 strain was grown in 60 μM fluconazole (red), 60 μM fluconazole + 40 μM 1181-0519 (blue), or no drug (black)