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Fig. 2 | Genome Biology

Fig. 2

From: Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design

Fig. 2

Parallel analysis of CRIPSRi-induced fitness defects in pooled cultures. a Effect of gRNA expression on growth. gRNA sequencing counts following growth in induced (+ATc) vs. uninduced (-ATc) conditions were used to calculate the ATc-effect (A0) for each gRNA, which were median-centered and plotted on the y-axis. Each point represents a gRNA directed against one of 20 different target genes (gene_tiling20 library). gRNAs are color-coded and arranged alphabetically on the x-axis by target gene. In the plot, A0 values below -4 were set to -4. b Effect of small molecules on detecting gRNA-induced growth defects. For each gene target (x-axis), the number of gRNAs inducing a growth defect (median-centered A < -1) in standard conditions, and in the presence of its paired reference small molecule is plotted on the y-axis (see legend). c Fluconazole-specific growth defects (y-axis) are plotted for each gRNA (x-axis), which are color-coded and arranged alphabetically by target gene. The drug/gene pair representing the reference chemical-genetic interaction is highlighted in gray. d Drug-specific effects for the ERG11 gRNA set in 25 different drug conditions (x-axis). Points are color-coded by condition. Large black dots represent the mean in each drug condition, and are colored red if >1 or if < -1. In c and d, drug-specific effect (D) values below -4 were set to -4. e Heatmap illustrating the average drug-specific effect for each guide set (y-axis), in each condition (x-axis). A guide set refers to the group of guides directed against the same gene. Drug-sensitivity is indicated in red, drug-resistance in blue. Previously defined chemical-genetic interactions are arranged on the diagonal and are outlined in green. Triangles above indicate cases where the same compound was assayed at increasing concentrations

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