Fig. 6

BRG1 interacts with GATA3, and is recruited at GATA3-bound chromatin domains. a Ty1-GATA3 was immunoprecipitated from MDA-MB-231 nuclear extracts with Ty1 antibody. Immunoblots were performed with the indicated antibodies. b Endogenous GATA3 was immunoprecipitated from T47D nuclear extracts with GATA3 antibody. Immunoblots were performed with the indicated antibodies. c Representative genomic locus showing the BRG1 enrichment in control, wild-type, and mutant GATA3 expressing cells. The highlighted region displays increased signals of BRG1 ChIP-seq in wild-type GATA3 expressing cells. d Read density heatmaps showing the signal enrichment of BRG1 ChIP-seq signals. The same classification and common peaks used in Fig. 6a are represented. e Metagene profiles of normalized BRG1 ChIP-seq tag density in G1, G2, and G3 are shown