Fig. 3

OLA1P2 affected the translocation of the phosphorylated STAT3 protein. a GSEA demonstrated enrichment of STAT3 target genes. The top of the panel shows the enrichment scores for genes associated with STAT3 signaling pathway targets. The black lines represent the 17 most highly correlated targets. The bottom of the panel shows the ranking scores (correlations of all genes associated with STAT3 signaling pathway targets with aspirin). b QRT-PCR analysis of OLA1P2 expression in the colon cancer cell line COLO205 transfected with lenti-OLA1P2, shRNA-OLA1P2, or negative controls. c Total STAT3 protein and phosphorylated STAT3 protein levels were analyzed by immunoblot in COLO205 cells transfected with lenti-OLA1P2 or shRNA-OLA1P2. d RNA FISH analysis of OLA1P2 localization in COLO205 cells. e QRT-PCR analysis of OLA1P2 localization. OLA1P2 was mainly present in the cytoplasm. U2 RNA was used as a positive control for nuclear RNA. f Phosphorylated STAT3 protein levels in the cytoplasm and nuclei of COLO205 cells transfected with lenti-OLA1P2 were analyzed by immunoblot analysis. g Phosphorylated STAT3 protein levels in the cytoplasm and nuclei of COLO205 cells transfected with shRNA-OLA1P2 were analyzed by immunoblot analysis. ***: P <0.001