Fig. 4

Minigene splicing reporter analysis of the human-specific splicing change of SUGT1 protein-coding Alu exon (Exon 6). a Schematic diagrams of the pI-11-H3 minigene splicing reporter and the wild-type/mutant minigene constructs of SUGT1 Alu exon. The nucleotide differences between human and chimpanzee are indicated by asterisks on the chimpanzee sequence. b Representative gel image for fluorescently labeled RT-PCR analyses in Hela cells using the SUGT1 Alu exon wild-type and mutant minigene constructs