Fig. 3

Reproducibility of de novo lncRNA and mRNA expression. a Study overview. Top: the granulocyte de novo transcriptome annotation was generated from 10 healthy donors. Bottom: seven donors were sampled at three time points spaced by ≥1 month (Additional file 2A) and RNA was sequenced to assess intra-individual (using three time points from one donor) and inter-individual (using samples from seven different donors) expression reproducibility. b Granulocyte intra-individual (top) and inter-individual (bottom) expression reproducibility for de novo annotated lncRNAs (green) and mRNAs (blue). Transcripts detectable (RPKM >0.2) at each of three time points or not detected (RPKM <0.2) at any time point in all seven donors show intra-individual reproducibility. Transcripts detectable in each of seven donors (average RPKM of three replicates >0.2) show inter-individual reproducibility. Five expression bins were used: (1) 0.5 < RPKM ≤1; (2) 1 < RPKM ≤2; (3) 2 < RPKM ≤4; (4) 4 < RPKM ≤8; and (5) RPKM >8 (n = transcript number per bin). Chromosomes X, Y were discarded